5.

EdU proliferation assay of SMMC-7721 cells with ETV4 interference treated with sorafenib or cisplatin. SMMC-7721 cells were analyzed with EdU cell proliferation analysis after transfection with NC, si-ETV4-2, or si-ETV4-3 for 24 h followed by treatment with DMSO, sorafenib (5 μmol/L) or cisplatin (5 μmol/L) for 48 h. The cells were photographed under an inverted microscope (×200). Data were analyzed using One-way ANOVA, and the error bars indicate SD (**P < 0.01, ***P < 0.0001)