8.

氯喹处理后对M2-CM与索拉菲尼共培养的SMMC-7721细胞凋亡和自噬相关蛋白表达的影响

Effect of sorafenib on the expression of apoptosis- and autophagy-related proteins and the influence of M2-CM after CQ treatment.A: Effects of M2-CM on the expressions of Bcl-2, Bax, p62, and LC3B in SMMC-7721 cells pretreated with CQ (10 μmol/L) and then treated with sorafenib (2.5 μmol/L) for 24 h; B: Standard quantitative analysis of Bcl-2/ Bax with Image J grayscale scanning; C: Standard quantitative analysis of LC3II/ LC3I with Image J grayscale scanning; D: Standard quantitative analysis of p62/β-actin with Image J grayscale scanning(n=3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.