1.
实验用各引物序列与片段大小
Sequence of primers for real-time PCR and the length of the products
| Gene | Forward (F) and Reverse (R) primer (5'-3') | Lenth (bp) |
| ICAM-1 | 182 | |
| F：5'-CCGGAAGGTGTATGAACTGA-3' | ||
| R：5'-GGCAGCGTAGGGTAAGGTT-3' | ||
| VCAM-1 | 141 | |
| F：5'-CGAAAGGCCCAGTTGAAGGA-3' | ||
| R：5'-GAGCACGAGAAGCTCAGGAGAAA-3' | ||
| E-selectin | 132 | |
| F：5'-CACTCAAGGGCAGTGGACACA-3' | ||
| R：5'-CAGCTGGACCCATAACGGAAAC-3' | ||
| MCP-1 | 143 | |
| F：5'-GATGCAATCAATGCCCCAGTC-3' | ||
| R：5'-TCCTTGGCCACAATGGTCTTG-3' | ||
| HO-1 | 153 | |
| F：5'-ATGACACCAAGGACCAGAGC-3' | ||
| R：5'-GTGTAAGGACCCATCGGAGA-3' | ||
| NQO-1 | 129 | |
| F：5'-GGTTTGAGCGAGTGTTCATAGG-3' | ||
| R：5'-GCAGAGAGTACATGGAGCCAC-3' | ||
| β-actin | 170 | |
| F：5'-AATCTGGCACCACACCTTCTAC-3' | ||
| R：5'-ATAGCACAGCCTGGATAGCAAC-3' |