Figure 5.

Short-term increased dietary salt load induced changes in the frequencies of classical and intermediate monocyte subpopulations in peripheral blood of healthy human subjects. (a) Representative dot plots are shown illustrating gating strategy, including exclusion of doublet cells using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis, gating on live cells based on fixable viability dye staining, and defining monocyte population based on side-scatter area (SSC-A) versus CD45 expression. Monocyte subsets were defined based on CD14 and CD16 expression as follows: CD14-CD16+ nonclassical monocytes, CD14+CD16+ intermediate monocytes, and CD14+CD16- classical monocytes (b). Average frequencies of monocyte subpopulations are shown on bar graphs at (c). Data are presented as mean ± SD and were analyzed by t-test; ^∗p < 0.05 was considered significant.