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. 2019 Sep 15;2019:4616034. doi: 10.1155/2019/4616034

Figure 2.

Figure 2

Effects of DR-Ab on Ang II-induced intracellular ROS generation in H9c2 cells. DR-Ab (2 μM) was given 30 min before treatment with Ang II (100 nM) for 48 h. (a–c) Representative immunofluorescence image (a) and group data (b, c) showing that DR-Ab decreased ROS generation caused by Ang II. Red: DHE staining (a, upper). Scale bar, 100 μm. Green: DCFH-DA staining (a, lower). Scale bar, 50 μm. n = 6. (d) Effect of DR-Ab on the protein level of two subunits of NADPH oxidase: p22phox and p47phox. n = 4‐6. ∗p < 0.05 versus control group, †p < 0.05 versus Ang II alone group.