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(a) K562/ADM cells were transiently transfected with three DRAM1 siRNAs or the siNC. Downregulation of DRAM1 was confirmed by both qPCR and western blot (n = 3; ∗P < 0.05). (b) Western blot was used to examine LC3II/I expression after K562/ADM cells were transfected with DRAM1-siRNA (n = 3; ∗P < 0.05). (c) CCK-8 assay was used to examine the cell viability after K562/ADM cells were transfected with DRAM1-siRNA and subsequently treated with 140 μg/ml of ADM for 48 h (n = 3; ∗P < 0.05).
