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. 2019 Jul 25;39(10):2049–2066. doi: 10.1161/ATVBAHA.119.312732

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© 2019 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 1. — Characterization of perivascular adipose tissue-derived mesenchymal stem cells (PV-ADSCs). A and B, Representative histograms of flow cytometry analysis of cultured PV-ADSCs and the percentage of indicated phenotypic markers (n=3). Gating is set for the IgG control to be between 0.5% and 1%. C, Immunofluorescent staining of the adipose tissue surrounding the mouse aorta with CD29, Sca-1 (stem cell antigen 1), PECAM1 (platelet and endothelial cell adhesion molecule 1), PLIN-1 (perilipin-1), and 4′,6-diamidino-2-phenylindole (DAPI; n=3). Arrows indicate the CD29⁺/Sca1⁺/PECAM1⁻/PLIN1⁻ cells. The border between End (endothelium), Med (media), Adv (adventitia), and perivascular adipose tissue (PVAT) is drawn with dashed line. Scale bar, 50 μm. Ctrl indicates IgG isotype control.

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