Table 2 –
Gene expression data from Experiment 1
| Target | Male | Female | ||||||
|---|---|---|---|---|---|---|---|---|
| Control | Adol shock only |
Acute Adult Swim |
Adol shock/ Adult swim |
Control | Adol shock only |
Acute Adult Swim |
Adol shock/ Adult swim |
|
| PVN | ||||||||
| c-Fos | 114.8 ± 25.6 | 125.3 ± 23.9 | 552.9 ± 87.4^ | 472.4 ± 28.8^ | 100.3 ± 6.2 | 138.7 ± 23.6 | 518.2 ± 62.2^ | 490.7 ± 86.1^ |
| IL-1β | 107.2 ± 14.5 | 97.1 ± 14.8 | 204.6 ± 22.8^ | 215.1 ± 19.1^ | 87.5 ± 6.1 | 67.3 ± 10.8 | 170.7 ± 27.3^ | 193.0 ± 18.1^ |
| IL-6 | 139.4 ± 45.0 | 217.3 ± 27.1 | 184.3 ± 57.5 | 141.1 ± 33.4 | 194.4 ± 21.7 | 210.2 ± 43.2 | 205.3 ± 43.2 | 215.4 ± 29.5 |
| CRH | 129.2 ± 29.6 | 188.9 ± 36.5 | 215.9 ± 36.7^ | 244.0 ± 42.6^ | 149.7 ± 33.7 | 183.6 ± 30.3 | 219.1 ± 47.2 ^ | 215.1 ± 40.2^ |
| Medial Amygdala | ||||||||
| c-Fos | 109.0 ± 15.0 | 90.2 ± 12.7 | 401.3 ± 52.9^ | 491.5 ± 52.9^ | 98.1 ± 5.4 | 103.9 ± 13.2 | 399.1 ± 18.3^ | 471.3 ± 18.9^ |
| Arc | 102.5±8.7 | 112.7±8.2 | 154.2±17.7^ | 191.4±28.3^ | 96.7±9.4 | 116.6±11.9 | 168.6±20.0^ | 190.6±20.6^ |
| Erg1 | 103.5±11.0 | 97.5±2.7 | 175.2±17.1^ | 216.5±12.8^ | 96.4±9.7 | 107.4±9.2 | 202.4±17.6^ | 219.1±18.8^ |
| c-Jun | 104.5±10.6 | 101.2±10.8 | 140.5±11.1^ | 138.1±8.3^ | 104.9±11.2 | 101.9±7.8 | 157.3±16.7^ | 141.6±8.7^ |
| IL-1β | 108.3 ± 17.6 | 91.2 ± 19.9 | 145.5 ± 20.6^ | 129.4 ± 12.4^ | 119.7 ± 31.0 | 76.0 ± 14.9 | 125.4 ± 19.5 ^ | 157.8 ± 22.8^ |
| IL-6 | 103.0 ± 8.4 | 105.3 ± 17.1 | 92.6 ± 10.6 | 76.6 ± 8.5 | 76.1 ± 15.1* | 102.1 ± 17.5* | 122.1 ± 24.0* | 115.2 ± 16.4* |
| CRH | 105.1 ± 12.3 | 117.2 ± 12.6 | 170.3 ± 30.0^ | 186.8 ± 19.6^ | 176.1 ± 32.1* | 179.6 ± 30.8* | 211.4 ± 16.4^* | 207.5 ± 23.1^* |
| Central Amygdala | ||||||||
| c-Fos | 104.8 ± 11.3 | 98.9 ± 15.1 | 435.5 ± 66.5^ A | 431.3 ± 42.1^ A | 89.1 ± 9.6 | 118.7 ± 16.9 | 319.7 ± 26.0^ | 393.0 ± 46.0^ |
| Arc | 105.4±14.0 | 88.5±17.8 | 141.9±18.4^ | 148.1±22.4^ | 81.2±14.6 | 97.3±13.0 | 130.2±21.6^ | 157.8±27.5^ |
| IL-1β | 101.8 ± 7.1 | 117.4 ± 9.6 | 174.6 ± 19.2 | 161.5 ± 20.3 | 111.8 ± 22.3 | 108.1 ± 20.1 | 125.5 ± 11.7 | 145.9 ± 21.7 |
| IL-6 | 105.1 ± 12.0 | 111.7 ± 14.9 | 104.4 ± 12.9^ | 120.2 ± 17.2^ | 118.2 ± 17.3* | 151.9 ± 15.6* | 178.9 ± 19.9^* | 179.0 ± 18.4^* |
| CRH | 104.0 ± 10.4 | 115.1 ± 16.8 | 130.7 ± 11.4 | 113.9 ± 14.9 | 106.5 ± 20.5 | 110.2 ± 16.6 | 111.2 ± 14.8 | 139.7 ± 26.5 |
| Ventral Hippocampus | ||||||||
| c-Fos | 104.2 ± 13.1 | 77.6 ± 9.0 | 395.6 ± 47..7 | 389.6 ± 31.7 | 100.7 ± 13.5 | 76.9 ± 7.6 | 256.4 ± 40.5 | 327.0 ± 67.4 |
| IL-6 | 107.8 ± 14.5ac | 91.9 ± 19.6ac | 82.1 ± 8.6a | 122.7 ± 17.8ac | 124.6 ± 18.8ac | 117.7 ± 13.9ac | 205.0 ± 21.7b | 132.2 ± 10.7c |
| GR | 100.9 ± 5.0 | 96.00 ± 5.5 | 95.1 ± 2.7 | 105.4 ± 6.5 | 107.2 ± 6.8 | 94.1 ± 3.9 | 102.7 ± 3.9 | 100.8 ± 4.3 |
| MR | 100.6 ± 4.2 | 80.4 ± 9.0 | 89.7 ± 7.7 | 91.8 ± 6.7 | 86.7 ± 9.4 | 85.5 ± 7.3 | 90.6 ± 6.8 | 90.4 ± 4.3 |
| Gilz | 101.3 ± 6.1 | 90.6 ± 7.0 | 108.1 ± 9.7^ | 114.5 ± 12.4^ | 93.6 ± 8.4 | 97.1 ± 10.3 | 131.9 ± 12.6^ | 122.5 ± 4.1^ |
| Sgk1 | 102.6±9.0 | 82.9±12.2 | 100.7±13.6 | 116.6±11.0 | 103.1±15.8 | 82.5±8.5 | 118.4±14.3 | 93.5±9.3 |
Note. Gene expression results from real-time RT-PCR after 30 min forced swim in adulthood. Bold indicates a main effect of adolescent footshock,
*
indicates a main effect of sex,
^
indicates a main effect of forced swim, and letters indicate a significant overall interaction, with individual letters indicating groups that are statistically different (i.e., different letters = statistical difference).