Figure 2.

(a) PCA (R ²X = 0.39, Q ² = 0.056) and (b) OPLS‐DA (R ²X = 0.376, R ²Y = 0.999, Q ² = 0.916) score plots based on the ¹H NMR spectra of metabolites in colonic digesta obtained from HFD (black circles) and AXD (red squares). Each symbol represents a mouse; ellipses represent 95% CIs on the basis of Hotelling's T² statistic. OPLS‐DA model development used 5 mice in HFD group and 5 mice in AXD group; therefore, score plots represent results from the PLS‐DA model. PLS‐DA model validation was performed by using the remaining 5 mice/group. OPLS‐DA, orthogonal projections to latent structures discriminant analysis