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. 2019 May 29;8(10):1107–1122. doi: 10.1002/sctm.18-0218

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© 2019 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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3% 1,4‐butanediol diglycidyl ether collagen type I scaffolds are more efficient for iOCT4 thymic epithelial cells (TECs) expansion after in vivo transplantation in athymic nude mice. (A): Images of transplanted animals were acquired by IVIS series preclinical in vivo system, 1 week (upper panel) or 2 weeks (lower panel) after scaffolds transplantation. Color scale is reported on the right side of the image. (B): Quantitative optical and computed tomography using IVIS series preclinical in vivo imaging system of mouse 5 (upper panel) or mouse 6 (lower panel), 1 week or 2 weeks after transplantation, respectively. Color scale is reported on the right side of the image. (C): Immunohistochemistry analyses for detection of CK5+ medullary TECs or CK8+ cortical TECs inside scaffolds of transplanted animals 2 weeks after transplantation in athymic nude mice. Inner and serial sections were chosen for the analysis. Scale bar: 200 μm, 100 μm for magnification on the right panel.