Fig. 3.

Hypothalamic astrogliosis in aged high-fat diet (HFD)-fed offspring. A: representative images of astrocytes identified by immunofluorescent detection of glial fibrillary acidic protein (GFAP) protein in coronal sections of hypothalamus obtained from 12-mo-old male and female offspring of HFD or HFD+BCAA dams. Scale bar: 100 µm. B: quantification of GFAP-staining intensity (error bars reflect means ± SE) in the arcuate nucleus of the hypothalamus (ARC) from male and female offspring (n = 6/group). *P < 0.001 control vs. HFD and HFD+BCAA), as assessed by two-way ANOVA followed by Newman-Keuls test. BCAA, branched-chain amino acid.