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. 2019 Jul 16;317(3):E548–E558. doi: 10.1152/ajpendo.00178.2019

Table 1.

PCR primer sequences

Gene Forward Primer (5-3) Reverse Primer (5-3) Species
IL-1β AAATGGAGACCTGGAATC CCTCTTACTTCACTGTCT Mouse
TNFα CTATGTCTCAGCCTCTTC CATTTGGGAACTTCTCAT Mouse
MCP-1 CAAGATGATCCCAATGAG TTGGTGACAAAAACTACA Mouse
CD68 TGTTCAGCTCCAAGCCCAAA GTACCGTCACAACCTCCCTG Mouse
EdnrA AAATTGTTTTCAGTCCTGCC ACTGGATACTCGTTCCATTC Mouse
EdnrB AGCTTCTGAGCTTTTTGTTG AATACAGAGCGATTGGATTG Mouse
18S TCAAGAACGAAAGTCGGAGG GGACATCTAAGGGCATCAC Mouse
GAPDH CCAGCTACTCGCGGCTTTA GAGGGCTGCAGTCCGTATTT Mouse
CD68 CAACTGGTGCAGACAGCCTA ACAGTCATTCCCTGTCCCCT Human
TNFα GACAAGCCTGTAGCCCATGT GGAGGTTGACCTTGGTCTGG Human
IL-1β TCGCCAGTGAAATGATGGCT GGTCGGAGATTCGTAGCTGG Human
β-Tubulin GCGTCTACTACAACGGAGG ACTCTGACCAAAGATGAA Human

GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.