Table 1.

List of primers and universal probes used in the real-time PCR

Gene	Forward	Reverse	UPL
PGC1α	CACACACAATCGCAGTCTCA	GGGTCATTTGGTGACTCTGG	6
Prdm16	CCGAGTGTGGGAAGACATTT	AAGGTTTGACGGTGCTGTG	88
FGF21	ACTGTGGGTCCCTGTGCT	GGAGTCAGGGATCGGATGT	11
Cidea	GCAGGCTCCTCCCAAGTC	CCCTGCAGCTTGCCTATCT	50
Cox2	CCCTCTTTAACCGTGAAAACA	GGGATTATGTAGGAGTCAAAGTTT	49
Elovl3	ACTGGATGGTTTGCCAGCTA	AGGATGATGAAGGCCGTGT	14
Dio2	TGACTCGGTCATTCTCCTCA	CAGTTTCACCTGCTTGTAGGC	69
UCP1	GGATCTCCGCTGGTGTAATG	CCTCAGTGGGTTGCCCTAT	52
NPR1	CAACGGGACTTCCCAAGAG	TGGGGATGTCAGGAGGTG	83
NPR2	TTTCCGTCCTTACAGAAAGCTG	ATGCGCCACAACATGCTA	17
NPR3	TCGCTATGACAGACCCAGAA	CGCATTTCAAAACGACCTTC	79
ADR1	TCCCCTGGCTCTATTACTCCT	CAGGACACAGACGATGGAGA	76
ADR2	CCCAAACATCTCCTTTGTGG	GCCCCCAAGAAGAATAATCC	2
IL-6	CTCCACAAGCGCCTTCTC	CGGGGTAGGGAAAGCAGTA	9
TNFα	GCCGTCTCCTACCAGACAAA	GGGTCTCCCTTTGGCAAG	53
β1R	GTCCTTCCTGCCCATCCT	AGCACTTGGGGTCGTTCT	141
β3R	CTTCGTGGCCAACGTGAT	GCGTAGCCCAGCCAGTTA	79
ATGL	GCCATGATGGTGCCCTAC	CCAGCAAGCGGATGGTAA	34

β1R, β-1 adrenergic receptor; β3R, β-3 adrenergic receptor; ADR, adiponectin receptor; ATGL, adipose triglyceride lipase; Cidea, cell death activator; Cox2, cyclooxygenase; Dio2, type II iodothyronine deiodinase; Elovl3, fatty acid elongase 3; FGF21, fibroblast growth factor; NPR, natriuretic peptide receptor; PGC1α, peroxisome proliferator-activated receptor γ coactivator 1α; Prdm16, PR domain containing 16; UCP1, uncoupling protein 1.