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. 2019 Jun 19;317(3):C492–C501. doi: 10.1152/ajpcell.00058.2019

Fig. 2.

Fig. 2.

Effect of aryl hydrocarbon receptor nuclear translocator-like protein-1 (BMAL1) on sodium-glucose cotransporter 1 (SGLT1) protein level in Caco-2 cells. A: differentiated Caco-2 cells were transduced with control shRNA (shControl), BMAL1 shRNA (shBMAL1), adenovirus (Adv)-Control, or Adv-BMAL1 for 72 h. Cells were collected, and soluble lysate was prepared. B: total proteins (20 µg per lane) of cell lysates were subjected to Western blot analysis with the indicated antibodies, and protein abundance is expressed as a percentage of shControl and Adv-Control, respectively. The data were quantified, and each bar represents the mean ± SE of 3 independent trials. *P < 0.05, significantly different from each Control.