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. 2019 Jun 19;317(3):C492–C501. doi: 10.1152/ajpcell.00058.2019

Table 1.

Oligonucleotide sequences of primers used

RT-PCR Primers
Forward (5′–3′) Reverse (5′–3′)
hPAX4 promoter GGTGATTTCAGGACCTCGGG TCCTCAAACAGTCTAGCGCG
hSGLT1 promoter CAGGACAGCTCTTACCTGCC GCAAAGAGGGAGGCTCCAAT
hSGLT1 promoter for E-box GCACCCATTTTACAAGCGGA CACAAAGGCCCACTGTACTC
hSGLT1 promoter for HNF4α GTCATGCCTCCTCTCTTGGT GCTATTTCCTTGCCCTGTCC
hSGLT1 promoter for Sp1 GAGTACAGTGGGCCTTTGTG GAGGGAGCAGGGCAGTAG
hSGLT1 promoter for HNF1α TACTGCCCTGCTCCCTCAAA TCCTTCCCTCTCGCCAGG
hHNF1α CAGCCCACCTACGTTTAATCATT CCAACTCCAAAATCAGGACCTA
hHNF1β GGCAATTGCACAAATGTCCTCT GGAGTCCTTGACATCGTGGG
hCDX2 CTCGGCAGCCAAGTGAAAAC CAGAGAGCCCCAGCGTG
hSGLT1 TGGTGCACAGATCAGGTCAT GGACGACACAGGCAATTTTT
hPAX4 TCCTTACAGCCCATTCCATC CACCCTCATGCAGCTGTCTA
hPAX6 AGTGAATCAGCTCGGTGGTG GTCTGATGGAGCCAGTCTCG
hSP1 TCATCCGGACACCAACAGTG ATTGGAAATCTTACCTGGGGCA
hSHP GCTTAGCCCCAAGGAATATGC TTGGAGGCCTGGCACATC
hDBP CCAGTTCCACGTATAGCTGAA CAGCATATGTAGGTCACATGA
hFOXO1 AACCTGGCATTACAGTTGGCC AAATGCAGGAGGCATGACTACGT
hFOXO2 GCCATGCACTCGGCTTCCAGTAT CAGCGCCCACGTACGACGAC
hGATA4 TCCCTCTTCCCTCCTCAAAT GTCCCATCAGCGTGTAAAGG
hGATA6 GCCTTGCCTGCTATGGAATA ACCTCATGAACCGACTCAGC
hGAPDH GAAAGCCTGCCGGTGACTAA TTCCCGTTCTCAGCCTTGAC

CDX2, caudal-type homeobox protein-2; DBP, D-box-binding proline- and acid-rich basic region leucine zipper (PAR bZIP) transcription factor; E-box, enhancer box; FOXO1 and FOXO2, forkhead box proteins O1 and O2, respectively; GATA4 and GATA6, GATA-binding proteins-4 and -6, respectively; h, human; HNF1α, HNF1β, and HNF4α, hepatocyte nuclear factors 1-α, 1-β, and 4-α, respectively; PAX4 and PAX6, paired-homeodomain transcription factors 4 and 6, respectively; SGLT1, sodium-glucose cotransporter 1; SHP, small heterodimer partner; SP1, Sp1 transcription factor.