Table 1.
RT-PCR Primers |
||
---|---|---|
Forward (5′–3′) | Reverse (5′–3′) | |
hPAX4 promoter | GGTGATTTCAGGACCTCGGG | TCCTCAAACAGTCTAGCGCG |
hSGLT1 promoter | CAGGACAGCTCTTACCTGCC | GCAAAGAGGGAGGCTCCAAT |
hSGLT1 promoter for E-box | GCACCCATTTTACAAGCGGA | CACAAAGGCCCACTGTACTC |
hSGLT1 promoter for HNF4α | GTCATGCCTCCTCTCTTGGT | GCTATTTCCTTGCCCTGTCC |
hSGLT1 promoter for Sp1 | GAGTACAGTGGGCCTTTGTG | GAGGGAGCAGGGCAGTAG |
hSGLT1 promoter for HNF1α | TACTGCCCTGCTCCCTCAAA | TCCTTCCCTCTCGCCAGG |
hHNF1α | CAGCCCACCTACGTTTAATCATT | CCAACTCCAAAATCAGGACCTA |
hHNF1β | GGCAATTGCACAAATGTCCTCT | GGAGTCCTTGACATCGTGGG |
hCDX2 | CTCGGCAGCCAAGTGAAAAC | CAGAGAGCCCCAGCGTG |
hSGLT1 | TGGTGCACAGATCAGGTCAT | GGACGACACAGGCAATTTTT |
hPAX4 | TCCTTACAGCCCATTCCATC | CACCCTCATGCAGCTGTCTA |
hPAX6 | AGTGAATCAGCTCGGTGGTG | GTCTGATGGAGCCAGTCTCG |
hSP1 | TCATCCGGACACCAACAGTG | ATTGGAAATCTTACCTGGGGCA |
hSHP | GCTTAGCCCCAAGGAATATGC | TTGGAGGCCTGGCACATC |
hDBP | CCAGTTCCACGTATAGCTGAA | CAGCATATGTAGGTCACATGA |
hFOXO1 | AACCTGGCATTACAGTTGGCC | AAATGCAGGAGGCATGACTACGT |
hFOXO2 | GCCATGCACTCGGCTTCCAGTAT | CAGCGCCCACGTACGACGAC |
hGATA4 | TCCCTCTTCCCTCCTCAAAT | GTCCCATCAGCGTGTAAAGG |
hGATA6 | GCCTTGCCTGCTATGGAATA | ACCTCATGAACCGACTCAGC |
hGAPDH | GAAAGCCTGCCGGTGACTAA | TTCCCGTTCTCAGCCTTGAC |
CDX2, caudal-type homeobox protein-2; DBP, D-box-binding proline- and acid-rich basic region leucine zipper (PAR bZIP) transcription factor; E-box, enhancer box; FOXO1 and FOXO2, forkhead box proteins O1 and O2, respectively; GATA4 and GATA6, GATA-binding proteins-4 and -6, respectively; h, human; HNF1α, HNF1β, and HNF4α, hepatocyte nuclear factors 1-α, 1-β, and 4-α, respectively; PAX4 and PAX6, paired-homeodomain transcription factors 4 and 6, respectively; SGLT1, sodium-glucose cotransporter 1; SHP, small heterodimer partner; SP1, Sp1 transcription factor.