Skip to main content
. 2019 Jul 3;317(3):C481–C491. doi: 10.1152/ajpcell.00150.2019

Fig. 4.

Fig. 4.

Activation of transient receptor potential mucolipin 1 (TRPML1) channel by sphingosine (Sph). A: Sph (20 µM) induced remarkable increases in GCaMP3 fluorescence in podocytes transfected with GCaMP3-ML1. On the other hand, Sph had no effects on GCaMP3 fluorescence in GCaMP3-ML1-expressing podocytes after pretreatment with verapamil (Vera; 20 µM). The application of ionomycin (1 µM) induced maximal responses. B: summarized data showing responses in podocytes transfected with GCaMP3-ML1 induced by Sph (n = 6 batches of cultures with total 25 cells). *P < 0.05 vs. control (Ctrl)-vehicle (Vehl). #P < 0.05 vs. Sph-Vehl.