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. 2019 Jul 12;33(10):10872–10888. doi: 10.1096/fj.201900767R

Figure 11.

Figure 11

Sirt6 binds to Nrf2 and antagonizes Nrf2-Keap1 interaction in cardiomyocytes. A) Sirt6 was immunoprecipitated (IP) from control and Sirt6-overexpressing cardiomyocytes, and the precipitated beads were analyzed by Western blotting. B) Nrf2 was IP from the control and Sirt6KD cells, and the presence of Keap1 on beads was analyzed by Western blotting. C) Western analysis showing expression levels of Nrf2 and Keap1 in the nuclear fraction of cells. Histone H3 served as loading control. D, E) Quantification of the Western blots. Cont, control; WCL, whole cell lysate. Means ± se; n = 4. *P < 0.01.