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Parkin knockdown protected against Dox-induced cardiomyocyte death. H9c2 cells were pretreated with parkin-targeted siRNA (siParkin) or control siRNA (siCon) for 24 h and then exposed to either Dox (750 nM) or saline for an additional 24 h. Cell death was determined by the cleavage of caspase-3 and PARP. Data are expressed as means ± se and were analyzed by 1-way ANOVA (n = 3).
