Figure 8.

DRP1 haploinsufficiency attenuated Dox-induced mitochondrial fragmentation. Adult ventricular cardiomyocytes were isolated from wild-type and DRP1^+/− mice, infected with AdDsRed, and treated with 3 µM Dox for 24 h. Mitochondrial morphology was analyzed using ImageJ. The major and minor axes as well as the size and number of mitochondria were calculated. At least 5 images (each containing 5–15 cells) were captured per treatment. WT, wild type. Data are expressed as means ± se and were analyzed by ANOVA. *P < 0.01 vs. control (n = 3).