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. 2019 Jul 9;33(10):10954–10965. doi: 10.1096/fj.201900377R

Figure 5.

Figure 5

SMAD2 and SMAD3 are necessary for NANOG-induced COL3 expression but SMAD3 overexpression is also sufficient for enhanced COL3 production. A) Protein expression of COL3 in LPZ and LPN hMSCs as a function of the time of TGF-β treatment. B, C) COL3 levels after blocking with SB431542 (SB43) or SMAD4 knockdown in LPZ and LPN hMSCs. D) COL3 levels after SMAD2 or SMAD3 knockdown in LPZ and LPN hMSCs. E) COL3 expression upon overexpression of SMAD2 or SMAD3 in LP hMSCs. DOX (1 μg/ml) was added in the medium to express NANOG or ZsGreen. *P < 0.05 compared to LPZ (n = 3 independent experiments). Data are normalized to LPZ-TGF-β1 (A) or LPZ (B–E) and presented as means ± sd, n = 3 independent experiments.