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. 2019 Jul 3;33(10):10819–10832. doi: 10.1096/fj.201900609R

Figure 4.

Figure 4

Lats1flox/flox;Lats2flox/flox;CYP19-cre granulosa cells appear to transdifferentiate into multiple cell lineages. A) Representative image of a Lats1flox/flox;Lats2flox/flox;CYP19-cre (mutant) ovary at 30 dpp. Inset: follicle-like structure containing a necrotic center. B) GJA1 (connexin 43) IHC on ovarian sections from 21-d-old mutant mice. Inset: new cell populations lack the gap junction protein GJA1. C) Representative image of a follicle-like structure containing abundant eosinophilic cytoplasmic granules (arrows) from 3-mo-old mutant mice. D) S-100 IHC on ovarian sections from 30-d-old mutant mice. Occasional cells (of the new cell populations) stain positive for S-100 (arrows). E) Representative image of mutant ovaries at 4 mo. Inset: osteoblasts (asterisk) and osteocytes (arrows) are clearly visible. F) ALPL staining. Inset: osteoblasts and osteocytes stain positive for ALPL. G) Representative image of cord-like structures. H) The cells lining the cord-like structures stain positive for SOX9. I) Representative image of mutant ovaries at 5 mo of age that are almost entirely composed of bone and bone marrow (inset). H&E, hematoxylin and eosin.