FIG 1.

Construction of an assay system for a functional evaluation of the T-N2G protein in the absence of sMaf proteins. (A) Structure of the T-N2G expression vector. A cDNA encoding FLAG-Nrf2 fused to MafG with a flexible polypeptide linker was inserted into the PiggyBac dual promoter vector. (B) Amino acid sequences of the T-N2G protein from the bZIP region of Nrf2 to that of MafG are shown. bZIP regions are underlined. Leucine residues of the Nrf2 leucine zipper motif are shown with a black background. The linker is boxed. (C) A schematic diagram showing that in the absence of sMaf proteins, the T-N2G protein can exert its function while the other CNC factors cannot.