FIG 2.

Establishment of immortalized sMaf-deficient MEFs. (A) Mating strategy for the production of sMaf triple knockout (F0G0K0) embryos. (B) Immunoblot analysis showing that large T antigen is expressed in both F0G2K0-T and F0G0K0-T cells. Lamin B1 was detected as a loading control. (C) Genotyping PCR with genomic DNA from F0G2K0-T (G2) and F0G0K0-T (G0) cells. P and N, positive and negative controls, respectively. Note that MafF and MafK wild-type (WT) alleles are not detected in either cell. The MafG WT allele was detected only in F0G2K0-T cells, while the MafG KO allele was detected only in F0G0K0-T cells. A closed arrowhead indicates specific PCR products. An open arrowhead indicates nonspecific products. (D) Growth curves of F0G2K0-T and F0G0K0-T cells. (E) Phase-contrast images of F0G2K0-T and F0G0K0-T cells.