Fig. 1.
Overview of the experimental design, tissue sampling and experimental end points (A). Mean serum/plasma glucose concentration (+SE) in rainbow trout fasted for 2 days and in rainbow trout 6 h after being refed either a NoCHO diet (devoid of carbohydrates) or a HighCHO diet (containing 30% carbohydrates) (B). Diets used were isoenergetic. Individual data are represented by dots, with open circles representing samples from individual fish that correspond to randomly selected liver samples (n = 4) used for next-generation sequencing (NGS). Two separate one-way ANOVAs followed by Tukey’s post hoc (P < 0.05) tests revealed a significant induction of hyperglycemia 6 h after refeeding a carbohydrate-rich diet, both in the full sample set (F2,27 = 74.89, P < 0.01), as well as in the randomly selected subset of 4 samples per group (F2,12 = 18.27, P < 0.01). Different letters indicate significantly different glucose concentrations between groups.