Table 3.

Correlation of expression of differentially regulated miRNAs and predicted targets involved in pathways of interest relevant to hepatic regulation of glucose metabolism

	miRNA	Significantly Differentially Expressed	Pearson Correlation Coefficient	Significance
Glucose metabolism
glut9 GSONMT00066217001	ssa-miR-222a-3p_R-1 ssa-miR-222a-3p_R-1 ssa-miR-222b-3p pol-let-7a-3p_R+1_1ss3AG pol-let-7a-3p_R+1_1ss3AG dre-miR-722_L-2 dre-miR-722_L-2 dre-miR-722_L-2	All groups (ANOVA) Fasted-HighCHO All groups (ANOVA) All groups (ANOVA) Fasted-NoCHO All groups (ANOVA) Fasted-NoCHO Fasted-HighCHO	0.20 0.15 0.33 −0.37 −0.63 0.42 0.04 0.50	n.s. n.s. n.s. n.s. n.s. n.s. n.s. n.s.
adp-gk GSONMT00073455001	ssa-miR-214-3p_L-1_1ss23AT ssa-miR-214-3p_L-1_1ss23AT ssa-miR-27d-5p ssa-miR-27d-5p ssa-miR-27d-5p ola-miR-27c-5p_L+1R+1 1ss12CA ola-miR-27c-5p_L+1R+1_1ss12CA ola-miR-101b-5p ola-miR-101b-5p ola-miR-101b-5p ssa-miR-30c-5p_R+1_1ss24TC dre-miR-125b-2-3p_L+1_2ss8GA11TC	All groups (ANOVA) Fasted-HighCHO All groups (ANOVA) Fasted-HighCHO NoCHO-HighCHO All groups (ANOVA) Fasted-HighCHO All groups (ANOVA) Fasted-NoCHO Fasted-HighCHO Fasted-NoCHO Fasted-HighCHO	0.34 0.33 0.84 0.96 0.95 0.83 0.98 −0.27 0.59 −0.71 −0.55 0.77	n.s. n.s. P < 0.01 P < 0.01 P < 0.01 P < 0.01 P < 0.01 n.s. n.s. P < 0.05 n.s. P < 0.05
Cell signaling
ir GSONMT00026581001	dre-miR-18b-5p_1ss11TC dre-miR-18b-5p_1ss11TC dre-miR-18b-5p_1ss11TC dre-miR-190a_R+1 dre-miR-190a_R+1 dre-miR-190a_R+1 ssa-miR-30e-5p ssa-miR-30e-5p ssa-miR-30e-5p ssa-miR-30c-5p_R+1_1ss24TC ssa-miR-30c-5p_R+1_1ss24TC ssa-miR-30a-5p ssa-miR-722-5p_L-1R+1	All groups (ANOVA) Fasted-HighCHO noCHO-HighCHO All groups (ANOVA) Fasted-HighCHO noCHO-HighCHO All groups (ANOVA) Fasted-HighCHO noCHO-HighCHO Fasted-NoCHO Fasted-HighCHO Fasted-HighCHO Fasted-HighCHO	−0.03 0.04 0.02 −0.18 −0.12 −0.33 −0.01 0.00 0.00 −0.20 −0.74 0.12 −0.04	n.s. n.s. n.s. n.s. n.s. n.s. n.s. n.s. n.s. n.s. <0.05 n.s. n.s.
Other pathways of interest
Autophagy
soga1  GSONMT00057063001	dre-miR-140-5p_R+1 dre-miR-140-5p_R+1 dre-miR-140-5p_R+1 dre-miR-214-5p_R+1 ssa-miR-214-3p_L-1_1ss23AT	All groups (ANOVA) Fasted-HighCHO NoCHO-HighCHO ANOVA Fasted-HighCHO	−0.80 −0.86 −0.77 0.07 0.34	P < 0.01 P < 0.01 P < 0.05 n.s. n.s.
Mitochondrial fusion
fis1  GSONMT00039888001	fru-miR-200a_R-1 fru-miR-200a_R-1 fru-miR-200a_R-1	All groups (ANOVA) Fasted-NoCHO NoCHO-HighCHO	0.20 −0.25 −0.18	n.s. n.s. n.s.
mfn1  GSONMT00027395001	dre-let-7d-5p dre-let-7d-5p dre-let-7d-5p dre-let-7c-5p_1ss19GC dre-let-7c-5p_1ss19GC dre-let-7c-5p_1ss19GC dre-let-7a ssa-miR-27d-5p ssa-miR-27d-5p ssa-miR-27d-5p ola-miR-27c-5p_L+1R+1_1ss12CA ola-miR-27c-5p_L+1R+1_1ss12CA fru-miR-7_R+2	All groups (ANOVA) Fasted-NoCHO Fasted-HighCHO All groups (ANOVA) Fasted-HighCHO NoCHO-HighCHO Fasted-HighCHO All groups (ANOVA) Fasted-HighCHO NoCHO-HighCHO All groups (ANOVA) Fasted-HighCHO Fasted-NoCHO	0.74 0.89 0.93 0.57 0.94 −0.21 −0.72 −0.20 −0.54 −0.40 0.16 0.51 0.70	P < 0.01 P < 0.01 P < 0.01 n.s. P < 0.01 n.s. P < 0.05 n.s. n.s. n.s. n.s n.s n.s
mfn2  GSONMT00004526001	ssa-miR-214-3p_L-1_1ss23AT ssa-miR-214-3p_L-1_1ss23AT ssa-miR-16c-5p_R-1 ssa-miR-16c-5p_R-1 fru-miR-152 fru-miR-152 fru-miR-152 ccr-miR-15a ccr-miR-15a ccr-miR-15a ssa-miR-17-5p_R-1 ssa-miR-17-5p_R-1 dre-miR-18b-5p_1ss11TC dre-miR-18b-5p_1ss11TC dre-miR-18b-5p_1ss11TC ssa-miR-15b-5p ssa-miR-15b-5p dre-miR-181b-5p_R+1 ssa-miR-101b-5p ssa-miR-29b-2-5p_L-2	All groups (ANOVA) Fasted-HighCHO All groups (ANOVA) noCHO-HighCHO All groups (ANOVA) Fasted-HighCHO noCHO-HighCHO All groups (ANOVA) Fasted-HighCHO noCHO-HighCHO All groups (ANOVA) Fasted-HighCHO All groups (ANOVA) Fasted-HighCHO noCHO-HighCHO All groups (ANOVA) noCHO-HighCHO Fasted-NoCHO Fasted-HighCHO Fasted-HighCHO	0.83 0.87 0.16 0.13 −0.57 −0.81 −0.53 −0.60 −0.89 −0.26 −0.54 −0.77 −0.53 −0.75 −0.40 −0.39 0.31 0.91 −0.75 −0.55	P < 0.01 P < 0.01 n.s n.s n.s P < 0.05 n.s. P < 0.05 P < 0.01 n.s. n.s. P < 0.05 n.s. P < 0.05 n.s. n.s. n.s. P < 0.01 P < 0.05 n.s.