Fig. 4.

Influence of swine coronary adipose conditioned medium on mouse aortic extracellular matrix proteins. Exposure to conditioned medium from swine heart failure (HF) coronary adipose decreased mouse aorta elastin content (A) and increased advanced glycation end products (AGE; B) compared with control (CON), HF continuous exercise trained (HF + CONT) and HF interval exercise trained (HF + IT). Aminoguanidine (AMG) prevented both reduced elastin level and AGE accumulation (n = 6/group). AU, arbitrary units. Data are means ± SE. *HF −AMG vs. CON ±AMG, HF+CONT ±AMG, and HF+IT ±AMG, P < 0.05; ^$−AMG vs. +AMG within group, P < 0.05. Data were analyzed with a 2-way ANOVA. Right: representative immunohistochemistry images of mouse aorta showing medial elastin and AGE alterations. Scale bars, 100 μm.