Table 2.
Gene-specific primers for RT-PCR amplification
| Transcript | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
| Hprt1 | TTCCTCCTCAGACCGCTTTTC | ATCACTAATCACGACGCTGGG |
| Nppa | TGAGCGAGCAGACCGATGAA | GAGACGGGTTGACTTCCCCA |
| Nppb | ACAAGAGAGAGCAGGACACCA | TAAGGAAAAGCAGGAGCAGAATCA |
| Myh6 | CTGAAGAGGCGGAGGAACAGG | TATTGTGGGATAGCAACAGCGA |
| Myh7 | CTCCCAGAACACCAGCCTC | CCGCCTCCTCCACCTCT |
| Col1a1 | AAGAACGGAGATGATGGGGAA | ATCCAAACCACTGAAACCTCT |
| Col3a1 | GAATGGTGGCTTTCAGTTCAG | GCAGTGGTATGTAATGTTCTGGG |
| Tgfb1 | CTGAACCAAGGAGACGGAATACA | AGGAGCAGGAAGGGTCGGT |
| Mmp2 | TGCCCAGAGACTGCTATGTCC | GCACACCACACCTTGCCATC |
| Gpx3 | GCTTCCCGTTCCAAATGAGC | GAAGGAGGCTGGTGGCATAG |
| Gpx1 | CCGGGACTACACCGAAATGA | ACCATTCACCTCGCACTTCT |
| Nqo1 | TCCAGAAACGACATCACAGGG | GGCACCCCAAACCAATACAA |
| Txnrd1 | CTCTCTTTATCCTCAGTGTGCTT | CCCGCCGCCCTATGA |
| Srxn1 | AGCAACCTCCTGATACCCCA | GAACGGAACCCCCTCATTCT |
| Hmox1 | CAACATTGAGCTGTTTGAGGAGC | GAGCGGTGTCTGGGATGAAC |
| Idh2 | GAGGCGTTGGTATGGGGATG | CGAAGCCCGAAATGGACTCG |
Col1a1 and Col3a1, collagen α-1(I) chain and collagen α-1(III) chain, respectively; Gpx1 and Gpx3, glutathione peroxidase 1 and 3, respectively; Hmox1, heme oxygenase 1; Hprt1, hypoxanthine phosphoribosyltransferase 1; Idh2, isocitrate dehydrogenase 2; Mmp2, matrix metalloproteinase-2; Myh6 and Myh7, myosin heavy chain 6 and 7, respectively; Nppa and Nppb, natriuretic peptide A and B, respectively; Nqo1, NAD(P)H quinone dehydrogenase 1; Srxn1, sulfiredoxin 1; Tgfb1, transforming growth factor-β1; Txnrd1, thioredoxin reductase 1.