Fig. 2.

Representative flow cytometry scatter plots (left) for splenic CD45⁺CD4⁺, CD45⁺CD4⁻CD11b⁺, CD45⁺CD4⁻CD11b⁺Ly6G⁺, and CD45⁺CD4⁻CD11b⁺Ly6G⁻Ly6C⁺ cells either unfixed or fixed with 1% PFA, or cold methanol or acetone, and their quantitative group data (right). Data were analyzed using repeated-measures 1-way ANOVA with Dunnet’s multiple comparisons test. *P <0.05, **P < 0.01, and ***P < 0.001 was considered significant (n = 5 in each group).