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. 2019 Jul 5;317(3):H561–H574. doi: 10.1152/ajpheart.00564.2018

Fig. 8.

Fig. 8.

Quantitative PCR (qPCR) detection of ryanodine receptors (RyRs) in rat mesenteric collecting lymphatic vessels. A: means show relative expression to GAPDH determined by qPCR and using the 2-ΔCt method in isolated rat mesenteric collecting lymphatics (N = 7 rats) and rat brain, skeletal muscle, and cardiac muscle (all N = 2 rats). B: gel of the single amplicon products of expected size from the qPCR reactions. Rat mesenteric collecting lymphatics were harvested (30 lymphatic vessels per rat), and total RNA (50 ng) was reverse transcribed into cDNA and qPCR performed with specific primer/probe sets for Ryr1 (Rn01545085_m1), Ryr2 (Rn01470303_m1), Ryr3 (Rn01486097_m1), and GAPDH (Rn01775763_g1). Reactions with no template were also run as controls (NTC). The gel shown is representative of three separate experiments using mesenteric lymphatic samples obtained from three different rats.