Skip to main content
. 2019 Sep 17;24(18):3379. doi: 10.3390/molecules24183379

Figure 2.

Figure 2

miR-152 inhibits 3T3-L1 preadipocyte proliferation. (A) The efficiency of miR-152 overexpression and knockdown. (B) The mRNA expression levels of CDK4, cyclin D1, cyclin E, and E2F3 were detected by qRT-PCR after 3T3-L1 cells transfected with the miR-152 mimic, the miR-152 inhibitor or the negative control for 36 h, respectively. (C) Cell proliferation were measured by a CCK-8 (Cell Counting Kit-8) assay after 3T3-L1 cells were transfected with the miR-152 mimic, the miR-152 inhibitor or the negative control. (D) After transfection with the miR-152 mimic, the miR-152 inhibitor or the negative control for 36 h, cells were fixed for 5-ethynyl-20-deoxyuridine (EdU) staining. (E) The proportion of EdU-positive cells are presented. Results are presented as means ± SD. n = 3. * p < 0.05; ** p < 0.01. Scale = 100 μm.