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. 2019 Sep 17;24(18):3379. doi: 10.3390/molecules24183379

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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miR-152 promotes 3T3-L1 preadipocyte differentiation. (A) The efficiency of miR-152 overexpression and knockdown during 3T3-L1 cell differentiation. (B) Intracellular lipid accumulation was measured by Oil Red O Staining on day eight after adipogenic induction. (C) The contents of triglycerides in terminally differentiated adipocytes. (D) The mRNA expression levels of PPARγ, C/EBPα, FABP4, SCD1, and ACC1 were detected by qRT-PCR after 3T3-L1 cells transfected with the miR-152 mimic, the miR-152 inhibitor or the negative control on day eight; Results are presented as means ± SD. n = 3. * p < 0.05; ** p < 0.01. Scale = 100 μm.