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. 2019 Apr 8;234(11):19895–19910. doi: 10.1002/jcp.28588

Figure 4.

Figure 4

CircFNEC3B reduced the expression of E‐cadherin. (a) MGC‐803 cells were transfected with circFNDC3B vector (OVER) or empty vector (EV) for 48 hr. Overexpression of circFNDC3B only inhibited the expression of E‐cadherin protein. (WT: wild‐type; EV: empty vector; OVER: overexpressing circFNDC3B). (b) BGC‐823 cells were transfected with siRNA (S1, S2) or negative control siRNA (NC) for 48 hr. Silencing of circFNDC3B upregulated the expression of E‐cadherin and inhibited the expression of N‐cadherin, Vimentin, and SNAI1 in protein. (WT: wild‐type; NC: negative control; siRNA: S1, S2) (c,d) MGC‐803 cells were transfected with circFNDC3B vector (OVER) or empty vector (EV) for 48 hr. BGC‐823 cells were transfected with siRNA (S1, S2) or negative control siRNA (NC) for 48 hr. qRT‐PCR verified that circFNDC3B did not affect the expression of ECAD (CDH1) mRNA, SNAI1 mRNA, VIM mRNA, and NCAD (CDH2) mRNA. (WT: wild‐type; EV: empty vector; OVER: overexpressing circFNDC3B; NC: negative control; siRNA: S1, S2). Data were expressed as the mean ± SEM and were analyzed by independent samples t test, n = 3. *p < 0.05, **p < 0.01, ***p < 0.0001. FNDC3B: fibronectin type III domain‐containing protein 3B; qRT‐PCR: quantitative reverse transcription‐PCR; SEM: standard error of the mean; siRNA: small interfering RNA [Color figure can be viewed at wileyonlinelibrary.com]