Figure 3.

Impact of AR antagonists on downstream target genes. (a) Inhibition of androgen target gene expression. Cells were treated with R1881 and AR antagonists, and RNA was extracted 20 hr later. Real‐time qPCR was performed with appropriate primers and the results were normalized to human cyclophilin A expression. (b) Reduction of AR binding at gene regulatory regions. Nuclei were purified from cells treated with R1881 and darolutamide, and immunoprecipitation performed using an AR‐specific antibody. AR occupancy was determined by qRT‐PCR using appropriate primers. [Color figure can be viewed at wileyonlinelibrary.com]