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. 2019 Sep 13;24(18):3335. doi: 10.3390/molecules24183335

Figure 3.

Figure 3

AF4 inhibits breast cancer cell motility and invasion. (A) Mitomycin C-treated MDA-MB-231 cells were cultured in wells containing cell culture inserts, which were removed at 0 h. After 24 h culture in the absence or presence of 20 µg/mL AF4 cultures were photographed. Representative images and mean % migration ± SEM of 3 independent experiments are shown. (B) Serum-starved MDA-MB-231 cells were treated with 20 µg/mL AF4 for 24 h. Mean % migration ± SEM through an 8 µm porous membrane and (C) mean % invasion ± SEM through a fibronectin-coated 8 µm porous membrane were determined as described in the Methods. (D) MDA-MB-231 cells were cultured for 24 h in the absence or presence of the indicated concentrations of AF4. Relative expression of MMP2 was determined using Western blot analysis. Equal protein loading was confirmed by probing for β-actin expression. Data shown are representative blots and mean % relative expression ± SEM. Statistical analysis of 3 independent experiments was performed using (A–C) Student’s t-test or (D) ANOVA and Tukey’s multiple comparisons test; * p < 0.01, ** p < 0.001.