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. 2019 Sep 13;24(18):3335. doi: 10.3390/molecules24183335

Figure 6.

Figure 6

AF4 suppresses MDA-MB-231 xenograft growth. MDA-MB-231 cells were xenografted into the left flank of non-obese diabetic severe combined immunodeficient (NOD-SCID) female mice and AF4 (0.5 mg/kg) or saline was injected directly into the resulting tumors every second day (day 1, 3, 5, 7 and 9, indicated by arrows) for 9 days. The control group consisted of 10 animals and the AF4 treatment group was 7 animals. (A) Mean tumor volume ± SEM was determined every second day. Statistical analysis was determined by Student’s t-test; * p < 0.05. Excised tumors from each treatment group at day 15 are shown. (B) At day 15 mice were euthanized and tumors were excised, fixed, and sectioned for staining with hematoxylin and eosin (H&E) and detection of CD31 expression by immunohistochemistry (IHC). Representative sections are shown; N denotes areas of necrosis and L denotes live cells.