Skip to main content
. 2019 Sep 4;24(18):3207. doi: 10.3390/molecules24183207

Figure 1.

Figure 1

Effects of platycodigenin (PLA) on Lipopolysaccharide (LPS)-induced NO and pro-inflammatory cytokines production in BV2 microglia. BV2 microglia were seeded in 96-well plates for 12 h, followed by treatment with PLA for 36 h (A) and the cell viability was assayed. BV2 microglia (2 × 105 cells/mL) were seeded in 48-well plates for 12 h, followed by pretreatment with PLA for 12 h and then LPS (100 ng/mL) was stimulated for another 24 h. NO release (B) was detected by Griess reagent method and concentrations of TNF-α (C), IL-6 (D), IL-1β (E) and IL-10 (F) were determined by ELISA kit. The data are shown as the mean ± SEM of three independent experiments. Each group has five replicates in the independent experiment. *p < 0.05, and ***p < 0.001 versus vehicle (Veh), Kruskal-Wallis one-way analysis of variance by ranks test and one-way analysis of variance and Dunnett’s post hoc test.