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. 2019 Sep 4;24(18):3207. doi: 10.3390/molecules24183207

Figure 7.

Figure 7

Effects of platycodigenin (PLA) on neurite outgrowth. Primary cultured cortical neurons were cultured for 2 d following treated with PLA (0.01–10 μM) or control (Cont, 0.1% DMSO) for another 4 d. The neurons were fixed and immunostained for β3-tubulin and MAP2. The lengths of the β3-tubulin positive neurites were measured. (A) Time schedule of the experiments. (B) Quantification of total neurite outgrowth and associated statistics. (C) Representative images of β3-tubulin and MAP2 positive neurites. Scale bar, 100 μm. The data are shown as the mean ± SEM of three independent experiments. Ten images were captured of each group in the independent experiment. *p < 0.05 and **p < 0.01 versus control (Cont), one-way analysis of variance and Dunnett’s post hoc test.