Table 4.

The composition of the gradient (A:B) for luteolin-7-O-glucoside.

Time (min)	Solution A	Solution B	Flow rate (mL/min)
0–10	5%→35%	95%→65%	0.8
10–15	35%→40%	65%→60%	0.6
15–18	40%	60%	0.8
18–20	40%→42%	60%→58%	0.8
20–25	42%→100%	58%→0%	0.8