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Staining of proliferation markers on unfrozen cultured autologous oral mucosa epithelial cell sheets (CAOMECS; control cell sheet) and CAOMECS frozen for 204 days using the Vitrification Procedures 1, 2, and 3. The expression of proliferating cell nuclear antigen and Ki67 was preserved in any vitrification solution, but DeltaNp63 expression was not preserved in the Vitrification Procedure 3. Scale bar is 100 μm [Colour figure can be viewed at wileyonlinelibrary.com]
