Figure 4.

IgG enhances MAIT cell activation in the presence of normal human serum. (a) Deposition of C3 and IgG molecules on formaldehyde‐fixed E. coli in the presence of normal human serum (NHS) or heat‐inactivated normal human serum (hiNHS). (b) Activation of MAIT cells by different amounts (bacteria per cell, BpC) of formaldehyde‐fixed E. coli (ATCC2592) in the presence or absence of NHS or hiNHS. Data are shown as mean ± s.e.m. of nine independent experiments. (c) Binding of IgG molecules to different amounts (1 × 10⁶, 5 × 10⁶ or 25 × 10⁶) to either formaldehyde fixed or living E. coli (strain ATCC25922) in the presence or absence of hiNHS or different concentrations of purified IgG (Intratect). Data are shown as mean ± s.e.m. of two independent experiments. (d) Activation of MAIT cells by either formaldehyde‐fixed or living E. coli in the presence or absence of hiNHS or different concentrations of purified human IgG (Intratect). Data are shown as mean ± s.e.m. of four independent experiments.