Figure 6.

IgG‐opsonization of E. coli enhances cytokine production of MAIT cells by THP‐1 cells, but not by BCLs. THP‐1 cells (a) or BCLs (b) were preincubated for 5 or 24 h in the absence or presence of IgG‐opsonized E. coli (E. coli‐IgG) or E. coli. Enriched CD8 T cells were co‐cultured for 20 h with preincubated THP‐1 cells or BCLs. MAIT cells, gated for as in Figure 1a, were analyzed for the expression of IFNγ or TNF. The graphs on the right show the percentages of MAIT cells expressing IFNγ or TNF. Data are representative of seven (THP‐1) or eight (BCLs) donors or shown as mean ± s.e.m. of seven (THP‐1) or eight (BCLs) donors pooled from two experiments.