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. 2019 Sep 5;24(18):3241. doi: 10.3390/molecules24183241

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TUNEL assay of breast cancer tissue derived from 4T1 cells. Untreated breast tumor (Panel A) compared to treated breast tumor (Panel B). Treated breast tumor showed significant apoptosis marked score 4 compared to untreated group. Apoptotic cells (green fluorescence). Nonapoptotic cells (red). Panel C: Citral-induced apoptosis in vivo. Each bar represents the apoptosis index expressed as mean ± SD (n = 6). Citral treatment caused a significantly higher percentage of TUNNEL-positive apoptotic cells. 20× magnification. The data were generated from six fields per slide, with four slides analyzed from each tumor, and 3 tumors examined from each group. (* P ˂ 0.01).