Figure 4.

Effect of IL‐4 on VEGF gene and protein expression in hASCs under normoxia or hypoxia. (a,b) VEGF gene expression. Hypoxia enhanced VEGF expression in hASCs at Day 2 and 7. (a) Under normoxia, IL‐4 (1 and 10 ng/ml) increased VEGF expression at Day 2. (b) IL‐4 (1 and 10 ng/ml) decreased VEGF expression under normoxia and hypoxia at Day 7. (c) VEGF protein concentration. Hypoxia enhanced VEGF protein concentration. IL‐4 (10 ng/ml) inhibited VEGF protein independent of the oxygen concentration at Day 7. Results are mean ± SD from n = 3 (duplicate wells of three independent experiments). *Significant effect of IL‐4 under normoxia or hypoxia, p < 0.05. hASC: human adipose stem cell; IL: interleukin; VEGF: vascular endothelial growth factor