Table 1.

List of primer sequences used for analysis of proliferation, osteogenic and angiogenic markers by hASCs by PCR

	Oligonucleotide sequences
Genes (human)	Forward	Reverse
TBP	5′‐GGTCTGGGAAAATGGTGTGC‐3′	5′‐GCTGGAAAACCAACTTCTG‐3′
GUSB	5′‐CGCACAAGAGTGGTGCTGAG‐3′	5′‐GGAGGTGTCAGTCAGGTATT‐3′
KI67	5′‐CCCTCAGCAAGCCTGAGAA‐3′	5′‐AGAGGCGTATTAGGAGGCAAG‐3′
RUNX2	5′‐ATGCTTCATTCGCCTCAC‐3′	5′‐ACTGCTTGCAGCCTTAAAT‐3′
COL1	5′‐TCCGGCTCCTGCTCCTCTTA‐3′	5′‐GGCCAGTGTCTCCCTTG‐3′
OC	5′‐AGCCACCGAGACACCATGAGA‐3′	5′‐CTCCTGAAAGCCGATGTGGTC‐3′
VEGF‐165	5′‐ATCTTCAAGCCATCCTGTGTGC‐3′	5′‐CAAGGCCCACAGGGATTTTC‐3′

Note. COL1: collagen type 1; GUSB: β‐glucuronidase; KI67: proliferation marker; OC: osteocalcin; RUNX2: runt‐related transcription factor‐2; TBP: TATA‐box binding protein; VEGF‐165: vascular endothelial growth factor.