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. 2019 Sep 14;24(18):3346. doi: 10.3390/molecules24183346

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CtsK and NFATc1 expression levels were decreased during osteoclastogenesis by PSTP-3,5-Me treatment. (A) mRNA expression levels of osteoclast-specific markers were determined by RT-PCR in 0 or 6 µM PSTP-3,5-Me-treated cells. *p < 0.05, and **p < 0.01 indicate the statistically significant difference between non-PSTP-3,5-Me-treated groups (0 µM) and PSTP-3,5-Me-treated groups (6 µM) on each day. Mean ± standard error. (B) Western blotting was performed to determine phosphorylation of NF-kB, p-38, ERK1/2, and JNK. Cells were pre-treated with 6 µM PSTP-3,5-Me or vehicle (Ctrl) for 2 h, and then treated with RANKL for the indicated times. β-Actin expression level was used as a loading control.