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Analytical HPLC conditions for C. tricuspidata fruit extracts.
| Parameters | Conditions | ||
|---|---|---|---|
| Instruments and Column | Alliance 2695 HPLC system (Waters, Millford, MA, USA) Zorbax extended-C18 (C18, 4.6 mm × 150 mm, 5 µm) |
||
| Flow rate | 0.8 mL/min | ||
| Injection volumn | 10 μL | ||
| UV detection | 330 nm | ||
| Run time | 30 min | ||
| Gradient | Time (min) | A (%) | B (%) |
| 0 | 10 | 90 | |
| 7 | 10 | 90 | |
| 8 | 20 | 80 | |
| 20 | 25 | 75 | |
| 21 | 100 | 0 | |
| 25 | 10 | 90 | |
| 30 | 10 | 90 | |
