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. 2019 Sep 25;63(3):3064. doi: 10.4081/ejh.2019.3064

Figure 1.

Figure 1.

Cell characterization. A) Cytofluorimetric analysis; −, negative expression (0%); +, moderate expression; ++, positive expression; +++, high expression (100%); MFI ratio is the average of three different biological samples ± standard deviation. B) Plastic adherent hGMSCs observed at inverted light microscopy. C) Alizarin Red S positive staining in hGMSCs culture. D) Oil Red O staining in hGMSCs culture, cells showed a lipid droplet at cytoplasmic level. E) RT-PCR of osteogenic related markers, RUNX2 and ALP. F) RT-PCR of adipogenic related markers, FABP4 and PPAR (undifferentiated vs differentiated). **P<0.01 was considered statistically significant. Scale bars: 20 μm.