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Cell proliferation and viability. A) MTT assay. (B) Trypan blue exclusion test. Treatment with AS 60 μg/mL and AS 90 μg/mL showed no statistical differences in terms of cell proliferation and viability after 24, 48 and 72 h of culture when compared to the CTRL group. DIFF OSTEO group showed a decrease in cell proliferation and viability when compared to all experimental groups (**P<0.01) at 48 and 72 h of culture. The results are expressed as mean ± SD.
