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Osteogenic differentiation. Alizarin Red S staining were used to evaluate the calcium depositions (red) in (A) CTRL, (B) AS 60 μg/mL, (C) AS 90 μg/mL and (D) DIFF OSTEO. E) Quantitative analysis of Alizarin Red S staining was performed by measuring the absorbance of Alizarin Red S. *BioR membrane. The results are expressed as mean ± SD (AS 60 μg/mL vs AS 90 μg/mL; 60 μg/mL vs DIFF OSTEO). **P<0.01 was recognized to be significant. Scale bars: 20 μm.
