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. 2019 Sep 25;63(3):3064. doi: 10.4081/ejh.2019.3064

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©Copyright: the Author(s), 2019

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Figure 7. — Expression of OPN. Immunofluorescence detection of OPN in CTRL(A1-A5), AS 60 μg/mL (B1-B5), AS 90 μg/mL (C1-C5) and DIFF OSTEO (D1-D5). OPN expression showed a higher expression in AS 90 μg/mL and DIFF OSTEO if compared to the AS 60 μg/mL. No significant differences have been demonstrated when compared AS 90 μg/mL and DIFF OSTEO groups. Red fluorescence, cytoskeleton actin; green fluorescence, specific marker; blue fluorescence, cell nuclei; grey scale, membrane observed at transmission light channel; merge pictures showed the overlapping of abovementioned channels. Scale bar: 10 μm.